电磁辐射通过转化生长因子-β-介导的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：阐述伽马射线是否可通过转化生长因子-β（TGF-β）-内源性的上皮细胞-间质叠加 (EMT)来促进恶性肿瘤的来袭迁移。可用分之一2Gy(60)Coγ线紫外线源自人类器官的6种恶性肿瘤，记录与EMT涉及的巨大变化，这有数分别来进行全像新科技，肽质印迹方法有，免疫荧光新科技，划痕试制和Transwell小室试制来掩蔽并检测细胞膜组织形态，EMT标有，来袭迁移能力也等。采用酶联免疫吸附法检测这些恶性肿瘤中的TGF-β肽高度，来进行特别抗病毒SB431542来评估TGF-β信号通路在伽马射线EMT中的的抑制作用。经过分之一为2Gy紫外线的恶性肿瘤中的实际上间叶细胞膜的表达，与实为紫外线组相比其上皮细胞标有减少，间叶细胞膜标有增加，同时其来袭转到能力也增强，TGF-β肽高度也提高。进一步发现由A549伽马射线诱导的EMT可通过对TGF-β信号抑制发生逆转。这些结果表明TGF-β内源性的EMT在伽马射线诱导增强恶性肿瘤来袭转到能力也中的起着关键抑制作用。

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