电离辐射通过转化生长因子-β-介导的上皮-间质转换来促使癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：阐述放射是否可通过转换成内皮细胞-β（TGF-β）-激活的上皮-间质转换 (EMT)来促进免疫系统的首当其冲迁移。使用增幅2Gy(60)Coγ线紫外光是从人类脑部的6种免疫系统，历史纪录与EMT无关的转变，这包括分别为了让显微镜应用，蛋白质印迹方依此，免疫荧光应用，划痕检验和Transwell小室检验来辨别并检测细胞一组织形态，EMT记号，首当其冲迁移控制能力等。采用酶联免疫吸附依此检测这些免疫系统当中TGF-β蛋白高度，为了让引人注意减缓剂SB431542来指标TGF-β瞬时通路在放射EMT当中的主导作用。经过增幅为2Gy紫外光的免疫系统当中存在间叶细胞的表达，与假紫外光一组相比较其上皮记号提高，间叶细胞记号增加，同时其首当其冲转移控制能力减弱，TGF-β蛋白高度也提高。进一步挖掘出由A549放射可借的EMT可通过对TGF-β瞬时减缓暴发逆转。这些最近TGF-β激活的EMT在放射可借减弱免疫系统首当其冲转移控制能力当中起着关键主导作用。

总编： lizexiu